Biology Department Faculty
Eric S. Folker
Associate Professor
Eric S. Folker
Ph.D.
Contact
Higgins Hall 578
Telephone: 617-552-1809
Email: eric.folker@bc.edu
ORCID 0000-0003-1619-7330
Education
- Ph.D., Notre Dame
Research Interests
Nuclear movement; Muscle development and disease pathogenesis
Department
Research
In most text books the cell nucleus is depicted as a static sphere occupying space near the center of the cell. This depiction is extremely misleading. The nucleus is a highly mobile organelle that occupies precise positions within each cell dependent on cell type, developmental stage, and cellular activity. Despite the conservation of dynamic nuclear movements amongst cell types and amongst species, the mechanisms of nuclear movement are only now emerging. Furthermore, little is known about how nuclear movement contributes to tissue development and function.
Muscle is a tissue type of special interest with regards to nuclear movement. Muscle cells are syncytial containing numerous nuclei, each of which undergoes several distinct movements. The end result is that most of the nuclei reside at the periphery of the muscle cell, and evenly spaced such that the distance between nuclei is maximized, with a small percentage of nuclei clustered at neuromuscular junctions. This precise positioning of nuclei in muscle cells is disrupted in patients with disparate forms of muscle disease, suggesting that proper movement and positioning of nuclei is essential to muscle development and function.
To understand how and why nuclei move, we utilize the model system Drosophila melanogaster. Drosophila muscle cells are remarkably similar to those of humans, but offer superior genetic, optical, and physiological tractability. We capitalize on these advantages to perform genetic screens, advanced imaging in the developing organism, and functional output assays to define the mechanisms of nuclear movement and the determine how nuclear movement impacts muscle architecture and function.
Selected Publications
- Collins MA*, Mandigo TR*, Camuglia JM, Vazquez GA, Anderson AJ, Hudson CH, Hanron JL, Folker ES. Emery-Dreifuss Muscular Dystrophy-linked genes and Centronuclear myopathy-linked genes regulate myonuclear movement by different mechanisms. Mol Biol Cell. 2017 Aug 15;28(17):2303-2317
- Auld AL, Folker ES. Nucleus-dependent sarcomere assembly is mediated by the LINC complex. Mol Biol Cell. 2016 Aug 1;27(15):2351-9.
- Navarro AP, Collins MA, Folker ES. The nucleus is a conserved mechanosensation and mechanoresponse organelle. Cytoskeleton (Hoboken). 2016 Feb;73(2):59-67.
- Azevedo M, Schulman VK, Folker E, Balakrishnan M, Baylies M. Imaging Approaches to Investigate Myonuclear Positioning in Drosophila. Methods Mol Biol. 2016;1411:291-312.
- Schulman VK, Folker ES, Rosen JN, Baylies MK. Syd/JIP3 and JNK signaling are required for myonuclear positioning and muscle function. PLoS Genet. 2014 Dec 18;10(12):e1004880.
- Folker ES, Baylies MK. Nuclear positioning in muscle development and disease. Front Physiol. 2013 Dec 12;4:363.
- Folker, ES, Schulman, VK, and Baylies, MK. (2014). Translocating myonuclei have distinct leading and lagging edges that require Kinesin and Dynein. Development, 141(2), 355-356.
- Chang, W., Folker, E.S., Worman, H.J., & Gundersen, G.G. (2013). Emerin organizes actin flow for nuclear movement and centrosome orientation in migrating fibroblasts. Molecular Biology of the Cell, 24(24) 3869-3880.
- Folker, E.S. and Baylies, M.K. (2013). Nuclear Positioning in Muscle Development and Disease. Fronteirs in Physiology, 4 (363).
- Folker, E. S., Schulman, V. K., & Baylies, M. K. (2012). Muscle length and myonuclear position are independently regulated by distinct Dynein pathways. Development (Cambridge, England), 139(20), 3827–3837.
- Schulman, V. K., Folker, E. S., & Baylies, M. K. (2013). A method for reversible drug delivery to internal tissues of Drosophila embryos. Fly, 7(3).
- Metzger, T., Gache, V., Xu, M., Cadot, B., Folker, E. S., Richardson, B. E., et al. (2012). MAP and kinesin-dependent nuclear positioning is required for skeletal muscle function. Nature, 484(7392), 120–124.
- Folker, E. S., Ostlund, C., Luxton, G. W. G., Worman, H. J., & Gundersen, G. G. (2011). Lamin A variants that cause striated muscle disease are defective in anchoring transmembrane actin-associated nuclear lines for nuclear movement. Proceedings of the National Academy of Sciences of the United States of America, 108(1), 131–136.
- Luxton, G. W. G., Gomes, E. R., Folker, E. S., Worman, H. J., & Gundersen, G. G. (2011). TAN lines: a novel nuclear envelope structure involved in nuclear positioning. Nucleus (Austin, Tex.), 2(3), 173–181.
- Luxton, G. W. G., Gomes, E. R., Folker, E. S., Vintinner, E., & Gundersen, G. G. (2010). Linear arrays of nuclear envelope proteins harness retrograde actin flow for nuclear movement. Science (New York, N.Y.), 329(5994), 956–959.
- Ostlund, C., Folker, E. S., Choi, J. C., Gomes, E. R., Gundersen, G. G., & Worman, H. J. (2009). Dynamics and molecular interactions of linker of nucleoskeleton and cytoskeleton (LINC) complex proteins. Journal of cell science, 122(Pt 22), 4099–4108.
- Zhu, Z. C., Gupta, K. K., Slabbekoorn, A. R., Paulson, B. A., Folker, E. S., & Goodson, H. V. (2009). Interactions between EB1 and microtubules: dramatic effect of affinity tags and evidence for cooperative behavior. The Journal of biological chemistry, 284(47), 32651–32661.
- Gupta, K. K., Paulson, B. A., Folker, E. S., Charlebois, B., Hunt, A. J., & Goodson, H. V. (2009). Minimal plus-end tracking unit of the cytoplasmic linker protein CLIP-170. The Journal of biological chemistry, 284(11), 6735–6742.
- Goodson HV, Folker ES. CLASPing the cell cortex. Dev Cell. 2006 Jul;11(1):4-5.
- Folker, E. S., Baker, B. M., & Goodson, H. V. (2005). Interactions between CLIP-170, tubulin, and microtubules: implications for the mechanism of Clip-170 plus-end tracking behavior. Molecular biology of the cell, 16(11), 5373–5384.