Biology Department Faculty
Douglas Warner
Director of Laboratories
Douglas Warner
Ph.D.
Contact
Higgins Hall 378
Telephone: 617-552-3552
Email: douglas.warner@bc.edu
ORCID 0000-0002-5395-8744
Education
- B.S., University of California at Davis
- Ph.D., Uniformed Services University of the Health Sciences
Courses
BIOL2040: Investigations in Molecular Cell Biology
BIOL2000: Molecules & Cells
BIOL4810: Advanced Research in Molecular Microbiology
Programs Advised
Department
Selected Publications
- O’Connor CM & Warner DM (2016), Investigations in Molecular Cell Biology, Eagle Print
- Warner DM, Duval V, Levy SB. (2013) The contribution of PmrAB to the virulence of a clinical isolate of Escherichia coli. Virulence, 4: 634-7
- Warner DM, Yang Q, Duval V, Chen M, Xu Y, Levy SB. (2013) Involvement of MarR and YedS in carbapenem resistance in a clinical isolate of Escherichia coli from China Antimicrob Agents Chemother. 57:1935-7
- Warner D.M. and Levy, S.B. (2012) SoxS increases the expression of the zinc uptake system ZnuACB in an Escherichia coli murine pyelonephritis model. Journal of Bacteriology 194:1177-85
- Warner D.M., Levy S.B. (2010) Different effects of transcriptional regulators MarA, SoxS and Rob on susceptibility of Escherichia coli to cationic antimicrobial peptides (CAMPs): Rob-dependent CAMP induction of the marRAB operon. Microbiology 156(2):570-8
- Warner D.M., Shafer W.M., and Jerse A.E. (2008) Clinically Relevant Mutations that Cause Derepression of the Neisseria gonorrhoeae MtrC-MtrD-MtrE Efflux Pump System Confer Different Levels of Antimicrobial Resistance and In Vivo Fitness. Molecular Microbiology 70(2): 462-78
- Shafer W.M., Folster J.P., Warner D.M., Johnson P.J.T., Balthazar J.T., Kamal N., and.Jerse A.E (2008) Expression of the MtrC-MtrD-MtrE Efflux Pump in Neisseria gonorrhoeae and Bacterial Survival in the Presence of Antimicrobials in National Institute of Allergy and Infectious Diseases, NIH, Volume 1: Frontiers in Research, Humana Press
- Garvin L.E., Bash M.C., Keys C., Warner D.M., Ram S., Shafer W.M., and Jerse A.E. (2008) Phenotypic and Genotypic Analyses of Neisseria gonorrhoeae Isolates that Express Frequently Recovered PorB PIA Variable Region (VR) Types Suggest Certain P1A Porin Sequences Confer a Selective Advantage for Urogenital Tract Infection Infection and Immunity 76(8): 3700-3709
- Warner D.M., Folster J.P., Shafer W.M., and Jerse A.E. (2007) Regulation of the MtrC-MtrD-MtrE efflux pump system modulates the in vivo fitness of Neisseria gonorrhoeae. Journal of Infectious Diseases 196(12):1804-1812
- Warner D.M., Folster J.P., Shafer W.M., and Jerse A.E. (2007) Regulation of the MtrC-MtrD-MtrE efflux pump system modulates the in vivo fitness of Neisseria gonorrhoeae. Journal of Infectious Diseases 196(12):1804-1812
Instructor of Record for the following courses:
BIOL2040: Investigations in Molecular Cell Biology
This intensive laboratory course applies a through line to each learned technique, so that while the student learns pivotal hands on skills (and their scientific principles) to make them viable in the biotechnology workforce, they are performing hypothesis driven experiments which yield a final manuscript, in the style of a scientific journal. Techniques include, aseptic technique, microscopy, molecular cloning, PCR and western blot. Students become adept at interpreting data, troubleshooting and communicating findings in oral, poster, and manuscript form. The semester long project uses the model organism Saccharomyces cerevisiae, to answer questions of the evolutionary relatedness of various biochemical pathways across species.
BIOL2000: Molecules & Cells
Foundational course required for Biology majors that introduce students to living systems at the molecular level of organization. Topics introduced in this course include basic cellular biochemistry, gene regulation, cellular organization and metabolism, cell signaling and genetics.
BIOL4810: Advanced Research in Molecular Microbiology
The bacterium Acinetobacter baumannii is an opportunistic pathogen commonly found in soil. Natural levels of antibiotic resistance make this organism a wonderful model for understanding the medically relevant issue of antibiotic resistance. In this course, students use bioinformatics to find uncharacterized genes in the genome of the organism, clone, mutate and overexpress the gene to characterize its contribution to antibiotic resistance and other pathogenic traits. The course is framed by this one-gene per student project to familiarize the student with methods of molecular genetics, particularly: bioinformatics, PCR design and troubleshooting, molecular cloning, microbiological culture techniques, and antibiotic susceptibility testing.