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John G. Boylan, Ph.D.
Director, NMR Center

Before joining Boston College in 1992, Dr. John Boylan earned both an A.B. degree in Biology and a Ph.D. in Physical Chemistry from the University of Missouri. His research was conducted in the Renal Division of Children’s Hospital in St. Louis and focused on motional studies of healthy and diseased renal membranes by NMR. He received postdoctoral training at Boston University School of Medicine in the Biophysics Department. His research into the role of lipids in cardiovascular pathology and the structure and function of tissue kallikrein inhibitors was funded through a National Research Service Award and later through an American Heart Association Research Fellowship.

Nuclear Magnetic Resonance (NMR) Center
John Boylan, Ph.D.
Director
john.boylan.1@bc.edu
617.552.4766
Merkert 101A


Thusitha Jayasundera, Ph.D.
Assistant Director, NMR Center

Dr. Jayasundera obtained a B.S. degree in Chemistry from UMass Boston in 2003 and a Ph.D. in Biophysical Chemistry from Yale University in 2009. After completing two Postdoctoral Research Associate positions, first at St. Jude Children’s Cancer Research Hospital and later at Brown University, he joined the Chemistry Department at Boston College in 2011. His research training has always been focused on the multi-faceted field of protein NMR, including extensive dynamics and interaction studies between proteins and small molecules as well as the three-dimensional structure determination of proteins using modern NMR methods.

Nuclear Magnetic Resonance (NMR) Center
Thusitha Jayasundera, Ph.D.
Assistant Director
jayasund@bc.edu
617.552.6984
Merkert 107


The Nuclear Magnetic Resonance Center

Five Varian high-field NMR spectrometers are maintained and operated in the Nuclear Magnetic Resonance Center in the Boston College Chemistry Department:  a 600 MHz VNMRS, a 500 MHz VNMRS, a 500 MHz INOVA, a 400 MHz VNMRS, and a 400 MHz MR-400.  All the spectrometers are equipped with pulsed-field gradients, variable-temperature controllers, ProTune® automatic probe tuning and matching accessories; and are running the most up-to-date Varian VNMRJ software on Dell T3400 computers with the Red Hat Linux Enterprise 3.1 operating system.

The NMR probes available in the Center allow NMR users to observe most spin-active nuclei and to perform nearly all state-of-the-art 1D, 2D, and multi-dimensional liquids NMR experiments.  A combination of on-line, automation queue, and open-access reservation schemes are used to ensure most efficient use of the instruments.  A 50-sample SMS robotic auto-sampler is installed on the 400 VNMRS spectrometer that runs continually in full automation.

Two additional Dell workstations running the same software as the spectrometers, and 2 windows-based workstations running a variety of data processing and molecular modeling software programs allow users to process their data remotely and prepare reports and presentation graphics in the facility.  Remote spectrometer access options (VNC) are also in place for teaching and maintenance purposes.  A large selection of printing and plotting devices are available in the facility, as are a variety of remote storage devices and back-up servers.


Useful NMR Documents (Link)


Complete NMR Center Description

VNMRS 600 (nmr12)
Manufactured by Varian NMR Inc. (serial number MR0904W032) and including a 11.745 Tesla 54 mm bore Varian Premium Shielded long-hold magnet, the spectrometer is driven by a Dell T3400 computer running Red Hat Enterprise Linux (RHEL) 5.1A and Varian’s VNMRJ 2.3A NMR software.  The 4-channel spectrometer (2 HB, LB and 2 LB) is equipped with a z-axis Performa IV pulsed field gradient, direct-drive architecture, ProTune automated tune and match for high and low-band probe channels, and variable temperature control.

The spectrometer has a 600 MHz 1H-19F/15N-31P PFG AutoX DB NB probe (serial number MR0902W004) and a 600 MHz 1H{13C/15N} 5mm PFG automation-enabled triple-resonance probe (serial number MR0902W001).


VNMRS 500 (nmr15)
Manufactured by Varian NMR Inc. (serial number MR0908W023) and including a 11.7 Tesla 54 mm bore Varian Premium Shielded long-hold magnet (serial number 21058), the spectrometer is driven by a Dell T3400 computer running Red Hat Enterprise Linux (RHEL) 5.1A and Varian’s VNMRJ 2.3A NMR software.  The dual channel spectrometer is equipped with a z-axis Performa IV pulsed field gradient, direct-drive architecture, ProTune automated tune and match for high and low-band probe channels, and variable temperature control.

The spectrometer has a 500 MHz1H-19F/15N-31P OneNMR PFG probe.


INOVA 500 MHz (nmr11)
Manufactured by Varian NMR Inc. (serial number UI500-S010837) and including an 11.744 Tesla 51 mm bore Oxford magnet (serial number 98619), the spectrometer is driven by a Dell T3400 computer running Red Hat Enterprise Linux (RHEL) 5.1A and Varian’s VNMRJ 2.2D NMR software.  The spectrometer is equipped with x, y, and z-axis pulsed field gradients, waveform generators, and variable temperature control.

Probes include a 5 mm Varian 500 SW/PFG (z-gradient) 50-202 MHz Broadband probe (serial number P003607), a 5mm Varian 500 ID/PFG (x, y, z-gradients) 50-202 MHz Indirect Detection probe (serial number P003833), and a Varian 500 gHX 50-202 MHz Nano-probe (serial number P003580).


VNMRS 400 (nmr14)
Manufactured by Varian NMR Inc. (serial number MR0907W004) including a 9.395 Tesla actively shielded Oxford 400AS magnet (serial number M02807), the spectrometer is driven by a Dell T3400 computer running Red Hat Enterprise Linux (RHEL) 5.1A and Varian’s VNMRJ 2.3A NMR software.   The dual channel spectrometer is equipped with a z-axis Performa IV pulsed field gradient, direct-drive architecture, ProTune automated tune and match for high and low-band probe channels, variable temperature control, and an SMS 50-slot auto-sampler.

Probes include a  5mm Varian g4NUC (1H/19F/13C/31P) Auto-NMR probe (serial number P008353), and a 1H-19F/15N-31P 5mm PFG AutoX DB NB 400 MHz probe (serial number MR0902W042).



The auto-sampler on the VNMRS 400 is a Varian SMS-50 which holds and processes up to 50 NMR samples. It runs in full automation mode under Varian's VNMRJ 2.3A software. Samples are submitted to a day queue (30 minutes maximum per sample) between 8:00 am and 8:00 pm and to a night queue (as long as need to a maximum of 12 hours per sample) between 8:00 pm and 8:00 am. experiments.


MR-400 (nmr13)
Manufactured by Varian NMR Inc. (serial number MR0905W019) and including a 9.395 Tesla 54 mm bore Varian Premium Shielded long-hold magnet, the spectrometer is driven by a Dell T3400 computer running Red Hat Enterprise Linux (RHEL) 5.1A and Varian’s VNMRJ 2.3A NMR software.  The dual channel spectrometer is equipped with a z-axis Performa IV pulsed field gradient, direct-drive architecture, ProTune automated tune and match for high and low-band probe channels, and variable temperature control.

The spectrometer has a 400 MHz1H-19F/15N-31P PFG AutoX DB NB probe.




The University completed a $1.5 million overhaul of the Center for Nuclear Magnetic Resonance in fall 2009, with support from the National Institutes of Health and the U.S. Department of Energy. Working in the facility are from left, the CNMR's Director, Dr. John Boylan, and Chemistry graduate students Jamie O'Brien (background), Laura Brozek (foreground), and David Moebius.
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Mass Spectrometry
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