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Morrissey College of Arts and Sciences

The Applied Biosystems 7500 Fast Real-Time PCR System

shared biology equipment

The Applied Biosystems 7500 Fast Real-Time PCR System's applications include gene expression analysis, pathogen quantitation, SNP genotyping, isothermal and +/– assays utilizing internal positive controls.

This system offers maximum performance in the minimum time. Fully optimized for Fast cycling, the 7500 Fast delivers high-quality results in as little as 30 minutes.

Real-Time PCR

Here are some features of the 7500 Fast Real-Time PCR System:

  • Specially designed Fast block ensures thermal uniformity at top speeds.
  • Faster ramp rates enable rapid results without compromising extension times or assay quality.
  • Fast optical plates ensure excellent precision in 10-30 uL reaction volumes.
  • Use TaqMan Fast Universal PCR Master Mix to run multiple Fast assays on one plate.
  • Finish more runs per day with the excellent performance equivalent to that offered by the 7500 System.

The Applied Biosystems High Resolution Melting (HRM) Software is available on the 7500 Fast Real-Time PCR System. It delivers precise results in a standard 96-well format to be used for mutation scanning and genotyping, and can run ChIP assay with an easy to follow workflow and minimal subjective data analysis steps.

The 7500 Fast Real-Time PCR System is located in room 452. For questions, please contact Hugh Cam.