1st dimension
The BioRad protein isoelectric focusing (IEF) is a convenient system for separation of proteins based on isoelectric point (pI). IEF strips of 11 cm and 24 cm can be accommodated in the system across the spectrum of pH ranges. Active rehydration and protein separation take 5-20 hrs, depending on the electrophoresis protocol.
2nd dimension
First dimension strips are loaded on the second dimension SDS-PAGE gels in the BioRad Protean II gel system, including a powersource. Precast gels are available for the second dimension. Runs typically complete in 1-2 hrs.
See BioRad "how-to" video here»
More info on 2D electrophoresis:
http://en.wikipedia.org/wiki/Two-dimensional_gel_electrophoresis