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Morrissey College of Arts and Sciences

Pulsed Field Gel Electrophoresis

shared biology equipment

CHEF-DR IIIThe CHEF-DR III system uses two leading technologies, CHEF (Clamped Homogeneous Electric Fields) and PACE (Programmable Autonomously Controlled Electrodes).

The system separates large and small DNA fragments with better resolution, speed, and accuracy, than initial pulsed field methods. DNA from 100 bases to over 10 megabases (mb) may be effectively resolved. Everything from Yeast Artificial Chromosome (YACs) to M13 inserts can be separated with a single instrument. Applications include top down and bottom up maping, electrophoretic karyotyping, analysis of tumor cell DNA rearrangements, mammalian DNA analysis, and testing for bacterial, yeast, and parasite train homogeneity.

The CHEF-DR III system uses high voltage and current, and should be operated with care at all times.

The CHEF-DR III system is located in rm. 590. For questions, please contact Dr. Marc-Jan Gubbels.