BioRad 2D Protein Electrophoresis
shared biology equipment
The BioRad protein isoelectric focusing (IEF) is a convenient system for separation of proteins based on isoelectric point (pI). IEF strips of 11 cm and 24 cm can be accommodated in the system across the spectrum of pH ranges. Active rehydration and protein separation take 5-20 hrs, depending on the electrophoresis protocol.
First dimension strips are loaded on the second dimension SDS-PAGE gels in the BioRad Protean II gel system, including a powersource. Precast gels are available for the second dimension. Runs typically complete in one to two hours.
The 2D gel electrophoresis is located in room 501. For questions, please contact Marc-Jan Gubbels.