research associate professor
Ph.D., Penn State College of Medicine
Fields of Interest
NeuroAIDS pathogenesis, HIV/SIV peripheral neuropathy, osteopontin, monocytes/macrophages.
My research has examined the pathogenesis of central nervous system (CNS) diseases, focusing on neuroAIDS resulting from human immunodeficiency (HIV) and the non-human primate equivalent simian immunodeficiency (SIV) infection. I have specifically been interested in the viral and cellular interactions that lead to the disruption of cellular function in neuroAIDS.
My doctoral dissertation research was performed at the Penn State College of Medicine under the guidance of Dr. Brian Wigdahl. During this time, I examined the molecular mechanisms involved in HIV-associated dementia. My focus was the interaction of the cellular protein C/EBP and viral protein R, Vpr, with the HIV long terminal repeat (LTR) and the effect of sequence variation on this interaction. The HIV LTR drives the expression for the entire viral sequence and changes in its interaction may alter replication and disease progression.
For my postdoctoral fellowship, I joined the laboratory of Dr. Howard Fox at the Scripps Research Institute and focused on immune regulation and induction of osteopontin in macrophages and their contribution to neurological disease. One of my main foci was the examination of the mechanisms of monocyte accumulation in the brain during neuroAIDS. This work was critical because monocytes are a major target of the HIV and SIV and also serve as carriers of virus into the brain, where they help maintain viral persistence. We have shown that osteopontin prevents monocyte recirculation and apoptosis and is highly increased in HIV-associated dementia.
My current research focus at Boston College uses these experiences, and I will continue utilizing the non-human primate model of neuroAIDS to examine the role of monocyte/macrophages as viral reservoirs, and the effect of drug therapies in clearing anatomic and histologic reservoirs. In addition, I am beginning to develop an animal model of HIV associated peripheral neuropathy to study the relative roles of virus and activated resident and circulating macrophages in producing dorsal root ganglion cell damage and/or damage to the long sensory tracts in the spinal cord.
9-30-2012 to 6-30-2017 NIH Research Project Grant Program (R01) NINDS R01 NS082116
"Monocyte/macrophage traffic and peripheral nerve pathogenesis"
total 5 years $1,978,284
Dr. Tricia Burdo, PI
1-1-2014 to 5-31-2016 Boston College's University's Institute on Aging
Aging Research Incentive Grant (ARIG)
"Immune Biomarkers of HIV and Aging"
total 3 years $57,236
Dr. Tricia Burdo, PI
1. Walker J, Beck G, Campbell JC, Miller AD, Burdo TH and Williams KC. Anti-α4 Antibody Blocks Monocyte/Macrophage Traffic to the Heart and Decreases Cardiac Pathology in a SIV Infection Model of AIDS. Journal of the American Heart Association. Accepted 6-15-15.
2. Burdo TH*, Walker J and Williams KC. Macrophage Polarization in AIDS: Dynamic interface between anti-viral and anti-inflammatory macrophages during acute and chronic infection. Clinical and Cellular Immunology 2015, 6:3 *corresponding author.
3. Poudrier J, Soulas C, Chagnon-Choquet J, Burdo T, Autissier P, Oskar K, Williams KC, Roger M. High expression levels of BLyS/BAFF by blood dendritic cells and granulocytes are associated with B-cell dysregulation in SIV-infected macaques. PLoS One. 2015 Jun 24;10(6):e0131513.
4. Lamers SL, Nolan DJ, Rife BD, Fogel GB, McGrath MS, Burdo TH, Autissier P, Williams KC, Goodenow MM, Salemi M. Tracking the emergence of host-specific SIV env and nef populations reveals nef early adaptation and convergent evolution in brain of naturally progressing Rhesus macaques.J Virol. 2015 Jun 3. pii: JVI.01010-15.
5. Jessica R. Lakritz, Ayman Bodair, Neal Shah, Ryan O’Donnell, Michael J. Polydefkis, Andrew D. Miller, and Tricia H. Burdo*. Monocyte traffic, dorsal root ganglion histopathology, and loss of intraepidermal nerve fiber density (IENFD) in SIV peripheral neuropathy, Am J Pathol. 2015 Jul;185(7):1912-23. *corresponding author
6. Caroline Soulas, Patrick J. Autissier, Tricia H. Burdo, Michael Piatak Jr, Jeffrey D. Lifson, and Kenneth C. Williams. Distinct Phenotype, Longitudinal Changes of Numbers and Cell-associated Virus in Blood Dendritic Cells in SIV-Infected CD8- lymphocyte Depleted Macaques. PLos ONE, 2015 April 27;10(4):e0119764.
7. Brian T. Nowlin, Tricia H. Burdo, Cecily Conerly Midkiff, Marco Salemi, Xavier Alvarez and Kenneth C. Williams. SIVE lesions are comprised of CD163+ macrophages present in the CNS during early SIV infection and SIV+ macrophages recruited terminally with AIDS. Am J Pathol. 2015 Jun;185(6):1649-65.
8. Kenneth C. Williams and Tricia H. Burdo. Neuro-AIDS, Immunopathogenesis of, Encyclopedia of AIDS, Editors: Thomas J. Hope, Mario Stevenson, Douglas Richman. Spring Science + Business Media, New York, March 17, 2015.
9. Janet Lo, Eric S. Rosenberg, Michael L. Fitzgerald, Suzanne Bazner, Ezinne J. Ihenachor, Alison Keenan, Victoria Hawxhurst, Jeffrey Wei, Tricia Burdo, Kenneth Williams, Mason W. Freeman, Steven K. Grinspoon, HDL-mediated Cholesterol Efflux Capacity is Improved by Treatment with Antiretroviral Therapy in Acute HIV-1 Infection, Open Forum Infect Dis. 2014 Dec 16;1(3):ofu108.
10. Jennifer H. Campbell, Eva-Maria Ratai, Patrick Autissier, Andrew G. MacLean, Nicole A. Renner, David J. Nolan, Samantha Tse, Andrew D. Miller, R. Gonzalez, Marco Salemi, Tricia H. Burdo, Kenneth Williams. Anti-α4 Antibody Treatment Suppresses Virus Traffic to the Brain and Gut Early, and Stabilizes CNS Injury Late in Infection. PLos Pathogens, 2014 Dec 11;10(12):e1004533.
11. Samantha L Strickland, Brittany D Rife, Susanna L Lamers, David J Nolan, Nazle MC Veras, Mattia CF Prosperi, Tricia H Burdo, Patrick Autissier, Brian Nowlin, Maureen M Goodenow, Marc A Suchard, Kenneth C Williams and Marco Salemi, Spatiotemporal Dynamics of SIV Brain Infection in CD8+ Lymphocyte-Depleted Rhesus Macaques with NeuroAIDS, Journal of General Virology 2014 Dec; 95 (Pt 12):2784-95.
12. Suman Srinivasa, Kathleen V. Fitch, Eva Petrow, Tricia H. Burdo, Kenneth C. Williams, Janet Lo, Hélène C.F. Cote and Steven K. Grinspoon. Soluble CD163 is Associated with Shortened Telomere Length in HIV-infected Patients, J Acquir Immune Defic Syndr. 2014 Dec 1;67(4):414-8.
13. Tricia H. Burdo* and Andrew D. Miller, Animal Models of HIV Peripheral Neuropathy, Future Virology, May 2014, Vol. 9, No. 5, Pages 465-474 *corresponding author
14. Joshua A. Walker, Megan L. Sulciner, Katherine D. Misgen, Andrew D. Miller, Tricia H. Burdo, Kenneth C. Williams, Elevated Numbers of CD163+ Macrophages in Hearts of SIV Infected Monkeys Correlates with Cardiac Pathology and Fibrosis, AIDS Res Hum Retroviruses. 2014 Jul;30(7):685-94.
15. Burdo TH, Weiffenbach A, Woods SP, Letendre S, Ellis RJ and Williams KC, Elevated sCD163 is a Marker of Neurocognitive Impairment in HIV Infection, AIDS. 2013 Jun 1;27(9):1387-95.
16. Burdo TH, Lackner A and Williams KC, Monocyte/macrophages and their role in HIV neuropathogenesis, Immunological Reviews, 2013 Jul; 254(1):102-13.
17. Fitch KV, Srinivasa S, Abbara S, Burdo TH, Williams KC, Eneh P, Lo J, Grinspoon SK. Noncalcified Coronary Atherosclerotic Plaque and Immune Activation in HIV-infected Women, J Infect Dis. 2013 Dec;208(11):1737-46.
18. Williams K and Burdo TH. Monocyte Mobilization, Activation Markers, and Unique Macrophage Populations in the Brain: Observations From SIV infected Monkeys Are Informative With Regard to Pathogenic Mechanisms of HIV Infection in Humans. Journal of Neuroimmune Pharmacol. 2012 Jun;7(2):363-71. PMID: 22167311
19. Burdo TH*, Orzechowski K, Knight HL, Miller AD, Williams KC. Dorsal root ganglia damage in SIV-infected rhesus macaques: An animal model of HIV-induced sensory neuropathy. Am J Pathol. 2012 Apr;180(4):1362-9. PMID: 22322298 *corresponding author
20. Strickland SL, Gray RR, Lamers S, Burdo TH, Huenink E, Nolan D, Nowlin B, Alvarez X, Midkiff C, Goodenow M, Williams K, Salemi M. Efficient Transmission and Persistence of Low Frequency SIVmac251 Variants in CD8-depleted Rhesus Macaques with Different Neuropathology. J Gen Virol May 2012 93 (Pt 5):925-938 PMID: 22302881
21. Subramanian S, Tawakol A, Burdo TH, Abbara S, Wei J, Vijayakumar J, Corsini E, Abdelbaky A, Zanni MV, Hoffmann U, Williams KC, Lo J, Grinspoon SK. Arterial inflammation in patients with HIV. JAMA. 2012 Jul 25;308(4):379-86. With Editorial Commentary. PMID: 22820791
22. Pereyra F, Lo J, Triant VA, Wei J, Buzon MJ, Fitch KV, Hwang J, Campbell JH, Burdo TH, Williams KC, Abbara S, Grinspoon SK. Increased Coronary Atherosclerosis and Immune Activation in HIV-1 Elite Controllers. AIDS. 2012 Nov 28;26(18):2409-12.
23. Zanni, M.V.*, Burdo TH*, Makimura H., Williams KC, Grinspoon S. Relationship between Monocyte/Macrophage Activation Marker Soluble CD163 and Insulin Resistance in Obese and Normal-Weight Subjects. Clin Endocrinol (Oxf). 2012 Sep;77(3):385-90. *Authors contributed equally. PMID: 22098563
24. Williams K, Hickey WF, Burdo TH and Soulas C. Mononuclear phagocytes. In: The neurology of AIDS. HE Gendelman, I Grant, IP Everall, HS Fox, HA Gelbard, SA Lipton, S Swindells (eds). 2012 Oxford university press.
25. Burdo TH, Lentz MR, Autissier P, Krishnan A, Halpern E, Letendre S, Rosenberg ES, Ellis RJ and Williams KC. Soluble CD163 made by monocyte/macrophages is a novel marker of HIV activity in early and chronic infection prior to and after anti-retroviral therapy. Journal of Infectious Diseases, 2011 Jul;204(1):154-63. PMC3105035
26. Strickland S, Gray R, Lamers SL, Burdo TH, Huenink E, Nolan DJ, Nowlin B, Alvarez A, Midkiff CC, Goodenow MM, Williams K, Salemi M. Significant Genetic Heterogeneity of the SIVmac251 Viral Swarm Derived From Different Sources. AIDS Research and Human Retoviruses, 2011 Dec;27(12):1327-32. PMID: 21524235
27. Hou Q, Barr TP, Getsios S, Gee LE, Vickers JV, Rodella LF, Burdo TH, Eisenberg E, Guha U, Wymer JP, McCarthy KC, Kessler JA, Lavker RM, Borsani E, Rice FL, Albrecht PJ, Differential Expression and Modulation of CGRP isoforms in Keratinocytes: Implications for Neuropathic and Inflammatory Pain Mechanisms. Pain. 2011 Sep;152(9):2036-51. PMID: 21641113
28. Burdo TH, Lo J, Abbara S, Wei J, Delelys ME, Preffer F, Rosenberg ES, Williams KC, Grinspoon S. Soluble CD163, a Novel Marker of Activated Macrophages, Is Elevated and Associated With Noncalcified Coronary Plaque in HIV-Infected Patients. J Infect Dis. 2011 Oct;204(8):1227-36. PMID: 21917896
29. Ratai E-M, Annamalai L, Burdo TH, Joo CG, Bombardier JP, Fell R, Hakimelahi R, He J, Lentz MR, Campbell J, Curran E, Halpern EF, Masliah E, Westmoreland SV, Williams KC, González RG, Brain creatine elevation and N-acetylaspartate reduction indicates neuronal dysfunction in the setting of enhanced glial energy metabolism in a macaque model of NeuroAIDS. Magn Reson Med. 2011 Sep;66(3):625-34. doi: 10.1002/mrm.22821. Epub 2011 Mar 4. PMID: 2138110
30. Ratai E-M, Pilkenton S, He J, Fell R, Bombardier JP, Joo C-G, Lentz MR, Kim W-K, Burdo TH, Autissier P, Annamalai L, Curran E, O’Neil S, Westmoreland SV, Williams KC, Masliah E, González RG CD8+ Lymphocyte Depletion without SIV Infection does not Produce Metabolic Changes or Pathological Abnormalities in the Rhesus Macaque Brain, J Med Primatol. 2011 Oct;40(5):300-9. PMID: 21463330
31. Campbell JH, Burdo TH, Autissier P, Bombardier JP, Westmoreland SV, Soulas C, González RG, Ratai E-M, Williams KC, Minocycline Inhibition of Monocyte Activation Correlates with Neuronal Protection in SIV NeuroAIDS, PLoS One. 2011 April 6. PMC307183
32. Soulas C, Conerly-Midiff C, Kim W-K, Burdo TH, Alaverz A, Lackner A, Williams KC Recently infiltrating MAC387+ monocyte/macrophages: a third macrophage population involved in SIV and HIV encephalitic lesion formation, American Journal of Pathology, 2011 May;178(5):2121-35. PMC3081227
33. Kim W.K., Sun Y., Do H., Autissier P., Halpern E., Piatak M., Lifson J.D., Burdo T.H., McGrath M.S., Williams K.C. Monocyte heterogeneity underlying phenotypic changes in monocytes according to SIV disease stage. J Leuko Bio, Leading Edge Article with editorial, 2010, 87:557-567. PMID: 19843579
34. Autissier P., Soulas C., Burdo T.H., Williams K.C. Evaluation of a 12-color flow cytometry panel to study lymphocyte, monocyte and dendritic cell subsets in humans. Cytometry Part A, Epub Jan 22 2010. PMID: 20099249
35. Burdo T.H., Soulas C., Orzechowski K., Button J., Krishnan A., Sugimoto C., Alvarez X., Kuroda M., Williams K.C. Increased monocyte turnover from bone marrow correlates with severity of SIV encephalitis and CD163 levels in plasma. PLoS Pathog. 2010 Apr 15;6(4):e1000842. with featured image PMID: 20419144
36. Ratai E-M, Bombardier JP, Joo C-G, Annamalai L, Burdo TH, Campbell J, Fell R, Hakimelahi R, He J, Autissier P, Lentz MR, Halpern E, Masliah E, Williams KC, Westmoreland SV, González RG, Proton Magnetic Resonance Spectroscopy Reveals Neuroprotection by Oral Minocycline in a Nonhuman Primate Model of Accelerated NeuroAIDS. PLoS One. 2010 May 7;5(5):e10523. PMID: 20479889
37. Autissier P, Soulas C, Burdo TH, Williams KC., Immunophenotyping of lymphocyte, monocyte and dendritic cell subsets in normal rhesus macaques by 12-color flow cytometry: clarification on DC heterogeneity. J Immunol Methods. 2010 Aug 31;360(1-2):119-28. PMID: 20600075
38. Williams, KC and T.H. Burdo. HIV and SIV infection: the role of cellular restriction and immune responses in viral replication and pathogenesis. APMIS. 2009 May; 117(5-6): 400-12. NIHMSID: NIHMS141154
39. Marcondes, M.C.G. Lanigan, C.M.S. Burdo, T.H., Watry, D.D. and H. S. Fox. Increased expression of monocyte CD44v6 correlates with the development of encephalitis in rhesus macaques infected with simian immunodeficiency virus. The Journal of Infectious Diseases, 2008 Jun 1; 197(11): 1567-76. NIHMSID: NIHMS59001
40. Marcondes, M.C.G., Sopper, S., Burdo, T.H., Sauermann, U., Watry, D.D., Zandonatti, M. and H. S. Fox. CD4 deficits and disease course acceleration can be driven by a collapse of the CD8 response in SIV-infected Rhesus Macaques. AIDS. 2008 Jul 31; 22(12): 1441-52. NIHMSID: NIHMS58806
41. Burdo, T.H., Ellis, R. J., and H. S. Fox. Osteopontin is increased in HIV-associated dementia. The Journal of Infectious Diseases. 2008 Sept 1; 198(5): 715-722. NIHMSID: NIHMS58807
42. Coutinho, A., Flynn, C., Burdo, T.H., Mervis, R.F. and H. S. Fox. Chronic Methamphetamine induces structural changes in frontal cortex neurons and upregulates frontal lobe type I interferon. Journal of Neuroimmune Pharmacology, 2008 Dec; 3(4): 241-5. NIHMSID: NIHMS58429
43. Marcondes, M.C.G., Burdo, T.H., Sooper, S., Huitron-Resendiz, S., Watry, D., Flynn, C., Zandonatti, M. and H. S. Fox. Enrichment and persistence of virus-specific CTL in the brain of simian immunodeficiency virus-infected monkeys is associated with a unique cytokine environment. J Immunol. 2007 May 1;178(9):5812-5819. PMID: 17442965
44. Burdo TH, Wood MR, Fox HS. Osteopontin prevents monocyte recirculation and apoptosis. J Leukoc Biol. 2007 Jun;81(6):1504-11. NIHMSID: NIHMS58808.
45. Burdo, T.H., Katner, S.N., Taffe, M.A., and H.S. Fox. Neuroimmunity, drugs of abuse, and neuroAIDS. Journal of Neuroimmune Pharmacology, 1(1), 41-49, March 2006. PMID: 18040790
46. Gaskill, P. J., Watry, D. D., Burdo, T. H., and H. S. Fox. Development and characterization of positively selected brain-adapted SIV. Virology Journal, 2(1): 44, May 12, 2005. PMCID: PMC1145188
47. Burdo, T.H., Marcondes, M.C.G., Lanigan, C.M.S., Penedo, M.C.T., and H. S. Fox. Susceptibility of Chinese rhesus monkeys to SIV infection. AIDS, 19(15), 1704-1706, Oct 14, 2005. PMID: 16184046
48. Burdo, T. H., Gartner, S., Mauger, D., and B. Wigdahl. Region-specific distribution of HIV-1 LTRs containing specific configurations of C/EBP site II in brains derived from demented and non-demented patients. Journal of NeuroVirology, 10 (suppl. 1) 7-14, 2004. PMID: 14982733
49. Burdo, T. H., Nonnemacher, M., Irish, B. P., Choi, C. H., Krebs, F. C., Gartner, S., and B. Wigdahl. High-affinity interaction between HIV-1 Vpr and specific sequences that span the C/EBP and adjacent NF-kB sites within the HIV-1 LTR correlates with HIV-1-associated dementia. DNA and Cell Biology, 23 (4) 261-269, 2004. PMID: 15142383
50. Hogan, T. H., Stauff, D. L., Krebs, F. C., Gartner, S. Quiterio, S., and B. Wigdahl. Structural and functional evolution of HIV-1 LTR C/EBP sites and their use as molecular markers for CNS disease progression. Journal of NeuroVirology, 9(1): 55-68, 2003. PMID: 12587069
51. Nonnemacher, M. R., Hogan, T. H., Quiterio, S., Wigdahl, B., Henderson, A., and F. C. Krebs. Identification of binding sites for members of the C/EBP transcription factor family in the simian immunodeficiency virus long terminal repeat. Biomedicine and Pharmacotherapy, 57(1):34-40, 2003. PMID: 12642035
52. Hogan, T. H., Nonnemacher, M. R., Krebs, F. C., Henderson, A. and B. Wigdahl. HIV-1 Vpr binding to HIV-1 LTR C/EBP cis-acting elements and adjacent regions is sequence-specific. Biomedicine and Pharmacotherapy, 57 (1): 41-48, 2003. PMID: 12642036
53. Quiterio, S., Grant, C., Hogan, T. H., and B. Wigdahl. C/EBP- and Tat-mediated activation of the HIV-1 LTR in CD34+ hematopoietic progenitor cells. Biomedicine and Pharmacotherapy, 57(1): 49-56, 2003. PMID: 12642037
54. Krebs, F. C., Hogan, T. H., Quiterio, S., Gartner, S., and B. Wigdahl. Lentiviral LTR-directed expression, sequence variation, and disease pathogenesis. HIV Sequence Compendium 2001, Kuiken CL, Foley B, Hahn B, Korber B, McCutchan F, Marx PA, Mellors JW, Sodroski J, and Wolinksy S, Eds. Theoretical Biology and Biophysics Group, Los Alamos National Laboratory, Los Alamos, NM, 10: 1-42, 2002.
55. Hogan, T. H., Krebs, F. C., and B. Wigdahl. Regulation of human immunodeficiency virus type 1 gene expression and pathogenesis by CCAAT/enhancer binding proteins (C/EBP) in cells of the monocyte/macrophage lineage. Journal of NeuroVirology, 8 (suppl. 2): 21-26, 2002. PMID: 12491147
56. Ross, H. L., Nonnemacher, M. R., Hogan, T., Quiterio, S. J., Henderson, A., Krebs, F., and B. Wigdahl. Interaction between CCAAT/enhancer binding protein and cyclic AMP response element binding protein 1 regulates human immunodeficiency virus type 1 transcription in cells of the monocyte/macrophage lineage. Journal of Virology, 75: 1842-1856, 2001. PMCID: PMC114094
57. McAllister, J. J., Philips, D., Millhouse, S., Conner, J., Hogan, T., Ross, H. L., and B. Wigdahl. Analysis of the HIV-1 LTR NF-kB-proximal Sp site III: Evidence for cell type-specific gene regulation and viral replication. Virology, 274: 262-277, 2000. PMID: 10964770
58. Doern, G.V., Brueggemann, A., Pierce, G., Hogan, T., Holley Jr., H.P. and A. Rauch. Prevalence of antimicrobial resistance among 723 outpatient clinical isolates of Moraxella catarrhalis in the United States in 1994-1995: results of a 30-center national surveillance study. Antimicrobial Agents and Chemotherapy, 40: 2884-2886, 1996. PMCID: PMC163641