imaging facility manager
Bret L. Judson
Imaging Facility Manager
525 Higgins Hall
B.S., 1995, State University of New York – Binghamton
M.A., 1999, State University of New York – Binghamton
My interests lie in the field of cell biology with particular emphasis on using microscopy-based approaches to solve questions. I have been broadly trained in various light- and electron-based microscopy techniques; this includes attendance at the Quantitative and Fluorescence Microscopy course at the Mount Desert Island Biological Laboratory, and the Analytical and Quantitative Light Microscopy course at Woods Hole.
For my graduate thesis work, I used a combination of light and electron microscopy (both SEM and TEM) to solve ultrastructural details in the coralline red alga Jania adhaerens.
Prior to coming to Boston College I managed three different imaging facilities, two at Cornell University (light and electron microscopy) and one at Arizona State University (light microscopy). In addition I taught a graduate level microscopy course while at Arizona State University.
Currently, at Boston College, I manage the imaging facility within the department of biology. The facility serves the entire Boston College community. In addition to managing the imaging facility I also teach the Advanced Lab in Cell Imaging course in both the Fall and Spring semesters. This course is for advanced undergraduates and graduate students. The course is a mixture of traditional lectures and hands on laboratory exercises. The culmination of the course is a student-selected project and presentation covering some of the instrumentation that we have discussed in class.
Judson BL 2012. Boston College Imaging Handbook. A resource for Boston College researchers providing instructions and protocols for both light and electron microscopy.
Judson BL 2011. Weill Institute Imaging Handbook. Light and Electron Microscopy Resources and Services for Weill Institute Laboratories. A manual providing microscope user instructions and protocols for both electron and light microscopy in yeast and mammalian cells. 58 pages.
Bechler ME, Doody AM, Ha KD, Judson BL, Chen I, Brown WJ 2011. The Phospholipase A2 Enzyme Complex PAFAH Ib Mediates Endosomal Membrane Tubule Formation and Trafficking. Molecular Biology of the Cell. 22(13): 2348-59.
David Teis, Suraj Saksena, Bret L. Judson and Scott D. Emr 2010. ESCRT-II nucleates two ESCRT-III filaments required for cargo sorting and the formation of MVB vesicles. EMBO Journal. 29(5): 871-883.
Judson BL, and Brown WJ 2009. Assembly of an Intact Golgi Complex Requires Phospholipase A2 (PLA2) Activity, Membrane Tubules, and Dynein-Mediated Microtubule Transport. Biochemical and Biophysical Research Communications. 389(3): 473-477.
Qi Wang, Marcos V. A. S. Navarro, Gary Peng, Evan Molinelli, Shih Lin Goh, Bret L. Judson, Kanagalaghatta R. Rajashankar, and Holger Sondermann 2009. Molecular mechanism of membrane constriction and tubulation mediated by the F-BAR protein Pacsin/Syndapin. Proceedings of the National Academy of Sciences. 106(31): 12700-12705.
William J. Brown, Helen Plutner, Daniel Drecktrah, Bret L. Judson, and William E. Balch 2008. The Lysophospholipid Acyltransferase Antagonist CI-976 Inhibits a Late Step in COPII Vesicle Budding. Traffic 9: 1-12.
Chambers K, Judson B, Brown WJ 2005. A unique lysophospholipid acyltransferase (LPAT) antagonist, CI-976, affects secretory and endocytic membrane trafficking pathways. Journal of Cell Science. 118(14): 3061-71.
Pueschel C, Judson B, Wegeberg S 2005. Decalcification during epithallial cell turnover in Jania adhaerens (Corallinales, Rhodophyta). Phycologia 44(2): 156-162.
Chan D, Strang M, Judson B, Brown WJ 2004. Inhibition of Membrane Tubule Formation and Trafficking by Isotetrandrine, an Antagonist of G-protein Regulated Phospholipase A2 Enzymes. Molecular Biology of the Cell 15(4): 1871-1880.
Hunt A, Abraham JL, Judson B, Berry CL 2003. Toxicologic and epidemiologic clues from the characterization of the 1952 London smog fine particulate matter in archival autopsy lung tissues. Environmental Health Perspectives 111(9): 1209-14.
Pueschel, C.M., B.L. Judson, J.E. Esken, and E.L. Beiter 2002. A developmental explanation for the differences of the Corallina- and Jania- types of thallus surfaces in articulated coralline red algae. Phycologia 41: 79-86.
Judson, B.L. and C.M. Pueschel 2002. Ultrastructure of trichocyte (hair cell) complexes in Jania adhaerens (Corallinales, Rhodophyta). Phycologia 41: 68-78.